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Miniaturizing siRNA Transfection Using Acoustic Droplet Ejection
25 Mar 2010: Large-scale, high-throughput siRNA experiments require a transfer system that can facilitate reliable and reproducible transfections in high-density well-plate formats, as well as effective delivery of the siRNA molecule. This application note demonstrates superior performance of the Echo liquid handler compared to more traditional liquid handling methods for standard, reverse and lipid-free transfection of siRNA molecules into mammalian cells.
Labcyte
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Recent articles: |
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Nanoliter High Throughput Quantitative PCR
18 Sep 2009: The hybrid approach described here combines the superior accuracy, precision and dynamic range of RT-PCR with the parallelism of a microarray in an array of 3072 real time, 33 nl polymerase chain reactions (RT-PCRs) the size of a microscope slide. RT-PCR is demonstrated with an accuracy and precision equivalent to the same assay in a 384-well microplate.
BioTrove
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Monitoring functional inhibition of a cell surface receptor using RNA interference
 08 Jul 2008: The CellKey™ System is a revolutionary label-free, cell-based assay platform that enables real-time measurements of all types of endogenous receptors. This application bulletin describes a straightforward and effective method for monitoring functional knockdown of an endogenous cell surface receptor using the CellKey™ System and transiently transfected small interfering ribonucleic acids (siRNAs).
Molecular Devices
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Visualising axon guidance phenotypes induced by RNAi in chicken embryos
23 Jun 2008: The ability to turn off a gene of interest in a temporally and spatially controlled manner is key to the analysis of gene function in developing organisms. However, the induction of temporally controlled loss-of-function phenotypes is very difficult. This application note demonstrates the use of fluorescence stereo microscopy for developmental functional gene analysis.
Olympus Europa Holding GmbH
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Optimization of Electroporation Conditions With the Gene Pulser MXcell Electroporation System
11 Dec 2007: This technical note demonstrates how the unique plate format of the Gene Pulser MXcell system allows researchers to quickly test and optimize all electroporation conditions.
Bio-Rad Life Sciences
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Cell-based Genome Wide RNAi Screening with the novel scan^R Screening Station
15 Oct 2007: The increasing need for scientific methods that allow large-scale functional studies of molecules in their natural environment is becoming more and more apparent. For this purpose Olympus have developed the scan^R Screening Station, on the basis of a custom built screening set-up.
Olympus Europa Holding GmbH
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Exiqon – miRCURY™ LNA Array - microRNA expression profiling on HS Pro™ Hybridization Station
08 Aug 2007: miRNA profiling assays based on microarray technology are a powerful method for simultaneously monitoring the expression of a large number of miRNA species through various sample collections. This application note demonstrates that with the miRCURY™ LNA Array, expression profiling of all human, mouse and rat miRNAs can be performed with high specificity, sensitivity and reproducibility.
Tecan
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Optimizing electroporation parameters for effective gene silencing in Jurkat cells
18 Apr 2007: The efficient transfer of siRNA into cells is a prerequisite for a successful gene silencing approach. This application note demonstrates how after determining the required electrical parameters (voltage and pulse length) and finding the appropriate amount of siRNA, the knockdown of Cdc2 gene expression in Jurkat cells could be shown.
Eppendorf
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Efficient Delivery of Dharmacon SMARTpool® siRNA Reagents in Hard-To-Transfect Cell Lines Using amaxa NUCLEOFECTOR® 96-well Shuttle® System
05 Feb 2007: The application note demonstrates the efficient nucleofection of Dharmacon SMARTpool siRNA reagents into hard-to-transfect Jurkat cells using the 96-well Shuttle. In addition the application note highlights critical parameters for establishing successful siRNA experiments.
Thermo Fisher Scientific
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Exploiting miRNAs for Endogenous Gene Knockdown
05 Feb 2007: MicroRNAs (miRNAs) are small RNAs derived from RNA polymerase II (PolII) transcripts that regulate the function of complementary genes in eukaryotic cells in a process that largely overlaps with the RNAi pathway. This poster reports on the construction of Pol II-driven miRNA-based RNAi vectors which are functionality superior to traditional shRNA.
Invitrogen Limited
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